The Mu Enhancer Is Functionally Asymmetric Both incis and in trans
نویسندگان
چکیده
منابع مشابه
The Mu enhancer is functionally asymmetric both in cis and in trans. Topological selectivity of Mu transposition is enhancer-independent.
Mu DNA transposition from a negatively supercoiled DNA substrate requires interaction of an enhancer element with the left (attL) and right (attR) ends of Mu. The orientation of the L and R ends with respect to each other (inverted) and with respect to the enhancer is normally inviolate. We show that when the enhancer is provided in trans as a linear fragment, the head to head orientation of th...
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15 صفحه اولThe wing of the enhancer-binding domain of Mu phage transposase is flexible and is essential for efficient transposition.
A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is accomplished by the assembly of a stable synaptic complex of MuA protein and Mu DNA. This initial critical step is facilitated by the transient binding of the N-terminal domain of MuA to an enhancer DNA ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m008523200